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探索建立鱼类寄生圆形碘孢虫(MyxobolusrotundusNemezek,1911,Myxosporea,Bivalvulida)完整孢子酶联免疫吸附试验(Enzyme linkedimmunosorbentassay,ELISA)检测的可行性,对丙酮、戊二醛、甲醛、多聚甲醛、乙醇等几种常用固定剂应用于此方法的效果进行评价,初步建立了针对鱼类黏孢子虫的完整孢子ELISA检测模式。结果显示,同比其他几种固定剂,2%的多聚甲醛、0 25%的戊二醛对圆形碘孢虫完整孢子ELISA有较好的效果,经二者固定后,最低可检出10个成熟的完整孢子,这为筛选鱼类黏孢子虫孢子表面抗原分子的特异性配体,如单克隆抗体及基因工程抗体或抗体片断,分析其表面分子性质奠定了基础。同时,讨论了该方法在鱼类黏孢子虫病原检测、属种鉴定、系统发育及黏孢子虫基础生物学研究等方面的潜在应用价值。
To explore the feasibility of using Enzyme linked immunosorbentassay (ELISA) for detection of parasitic circular iodine sporozoites in fish (Myxobolus rotundus Nemezek, 1911, Myxosporea, Bivalvulida) and to explore the feasibility of using acetone, glutaraldehyde, formaldehyde, paraformaldehyde, Ethanol and other commonly used fixatives in the evaluation of the effectiveness of this method, the initial establishment of a complete spore ELISA detection of fish Mycoplasma sp. The results showed that compared with the other several fixatives, 2% paraformaldehyde and 0 25% glutaraldehyde had a good effect on the complete sporulation ELISA of circular iodine spore-pollination. After the two immobilized, the lowest detectable 10 A mature complete spore, which for the screening of Mycoplasma sp. Surface antigen molecules specific ligands, such as monoclonal antibodies and genetically engineered antibodies or antibody fragments, analysis of the surface molecular properties of the foundation. At the same time, the potential application value of this method in the detection of the pathogen of fish Myxosporidiosis, the identification of species, the phylogeny and the basic biology of Myxosporidium were discussed.