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背景已发现细胞凋亡在缺血再灌注损伤中起重要作用。普罗布考除可降低血清低和高密度脂蛋白胆固醇浓度,有效治疗高胆固醇血症外,还有抗氧化活性,抑制低密度脂蛋白胆固醇的氧化修饰,减少其在血管壁沉积的作用。还有研究证明普罗布考可改善左心室功能、防止左心室扩张和减少心肌纤维化。但普罗布考保护心肌作用的确切机制尚未清楚。目的探讨普罗布考对新西兰白兔缺血再灌注心肌细胞凋亡的作用及其可能机制;并探讨其对血清超氧化物歧化酶(serumsuperoxidedis-muase,SOD)和丙二醛水平的影响。设计随机对照的实验研究。地点、材料和干预本实验在广西医科大学医学科学实验中心完成。实验选用30只雄性新西兰白兔,白兔被随机分成3组假手术组、对照组和预治疗组,每组各10只。对照组(标准兔饲料饲养)和预治疗组(标准兔饲料饲养+普罗布考每日每只1000mg灌胃)各4周后建立缺血再灌注模型;假手术组(标准兔饲料饲养)4周后开胸用4-0号线穿过冠状动脉左前降支近端,但不予结扎。测定3组白兔心肌细胞凋亡指数、血清丙二醛、SOD水平。主要观察指标①普罗布考对心肌细胞凋亡的影响。②普罗布考对血清SOD和丙二醛水平的影响。结果对照组凋亡指数[(34.75±3.20)%]、血清丙二醛水平[(2.70±0.64)μmol/L]显著高于假手术组[(0.48?
Background It has been found that apoptosis plays an important role in ischemia-reperfusion injury. Probucol in addition to lowering serum low and high density lipoprotein cholesterol, effective treatment of hypercholesterolemia, but also antioxidant activity, inhibit the oxidation of low-density lipoprotein cholesterol, reducing its deposition in the vascular wall. There are studies have shown that probucol can improve left ventricular function, prevent left ventricular dilatation and reduce myocardial fibrosis. However, the exact mechanism by which probucol protects myocardium is not yet known. Objective To investigate the effect and mechanism of probucol on myocardial cell apoptosis induced by ischemia-reperfusion in New Zealand White rabbits and to explore its effect on serum superoxide dismutase (SOD) and malondialdehyde (MDA) levels. Design of Randomized Controlled Experimental Study. Location, Materials and Intervention This experiment was completed in Guangxi Medical University Medical Science Experimental Center. Thirty male New Zealand white rabbits were selected in the experiment. The rabbits were randomly divided into three groups: sham operation group, control group and pretreatment group, 10 in each group. The ischemia-reperfusion model was established in the control group (standard rabbit feeding) and the pre-treatment group (standard rabbit feeding + probucol daily 1000mg) Thoracotomy with a 4-0 line through the left anterior descending coronary artery proximal, but not ligation. The apoptosis index, serum malondialdehyde and SOD of the three groups were measured. MAIN OUTCOME MEASURES ① The effects of probucol on cardiomyocyte apoptosis. ② probucol serum SOD and malondialdehyde levels. Results The apoptosis index (34.75 ± 3.20)% and the serum malondialdehyde level (2.70 ± 0.64 μmol / L) in the control group were significantly higher than those in the sham group [(0.48?