论文部分内容阅读
目的:建立HPLC同时测定炎热清软胶囊中栀子苷、黄芩苷和汉黄芩苷的含量。方法:样品采用石油醚回流脱脂,70%甲醇超声提取30 min。采用Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.4%磷酸溶液梯度洗脱,流速1.0 m L·min-1,柱温30℃,检测波长240 nm。结果:炎热清软胶囊中3种有效成分的分离度良好,栀子苷、黄芩苷和汉黄芩苷的平均回收率分别为100.63%,100.77%和97.67%,RSD分别为1.9%,1.5%和1.1%,(n=6)。结论:该方法简便准确、灵敏度高、专属性强,重复性好,可用于炎热清软胶囊的质量控制。
OBJECTIVE: To establish HPLC simultaneous determination of geniposide, baicalin and baicalin in Yanreqing soft capsule. Methods: The samples were degreased with petroleum ether and extracted with 70% methanol for 30 min. Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was eluted with a gradient of acetonitrile-0.4% phosphoric acid. The flow rate was 1.0 m L · min-1. The column temperature was 30 ℃ and the detection wavelength was 240 nm. Results: The resolution of three active ingredients in YRF capsules was good. The average recoveries of geniposide, baicalin and baicalin were 100.63%, 100.77% and 97.67%, respectively, and the RSDs were 1.9%, 1.5% and 1.1%, (n = 6). Conclusion: The method is simple and accurate, high sensitivity, specificity and repeatability, and can be used for quality control of the capsule.