用免疫组织化学PAP法,对小鼠脑垂体TSH细胞、ACTH细胞进行双重染色,在同一切片上同时显示两种细胞,观察细胞形态,并用体视学方法对两种细胞进行定量测定。结果显示,TSH细胞体积较大,呈多边形;ACTH细胞体积略小,呈不规则形。TSH细胞的体积密度、面数密度、数密度均高于ACTH细胞的相应数值,而TSH细胞的相邻细胞百分比则低于ACTH细胞的相应数值。 Immunohistochemical PAP method was used to double staining TSH and ACTH cells in the pituitary of the mice. Two kinds of cells were simultaneously displayed on the same slice. Morphology of the cells was observed. The two kinds of cells were quantitatively determined by stereological methods. The results showed that, TSH cells larger, polygonal; ACTH cells slightly smaller, irregular shape. The density, density and density of TSH cells were higher than that of ACTH cells, while the percentage of adjacent cells of TSH cells was lower than that of ACTH cells.