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目的 :寻找新的肝细胞癌 (hepatocellularcarcinoma ,HCC)相关基因。方法 :应用荧光 差异显示 (fluorescentdifferentialdisplay)技术分析人HCC、配对非癌肝、胎肝、正常肝等组织之间的基因差异表达 ,差异表达cDNA片段 ,经测序后 ,再用RNA狭缝杂交对部分差异片段在上述 4种组织中的表达进行验证。结果 :共得到差异表达cDNA片段 110条。经狭缝杂交筛选 ,获得了 4条阳性片段 ,未知cDNA片段L2和与人醛缩酶B 99.5 %同源的cDNA片段L7在部分HCC中特异性低表达 ,未知cDNA片段LC2 7和与人Nip3基因 99.4%同源的cDNA片段LC2 0在部分HCC中特异性高表达。结论 :确认了 4条HCC相关基因。其中L2和人醛缩酶B基因可能为阻抑HCC发生、发展的基因 ,而人Nip3基因和LC2 7可能为促进HCC发生、发展的基因。L2和LC2 7为目前未知的新基因。
Objective: To search for new hepatocellular carcinoma (HCC) related genes. METHODS: Differential gene expression was performed between human HCC, matched non-cancerous liver, fetal liver, normal liver, and other differentially expressed cDNA fragments using fluorescence differential display (Diffractive Differential Display). After sequencing, RNA Slit Hybridization The differential fragments were verified in the above four tissues. Results : A total of 110 differentially expressed cDNA fragments were obtained. After screening by slit hybridization, four positive fragments were obtained. The unknown cDNA fragment L2 and the cDNA fragment L9, which is 99.5 % homologous to human aldolase B, were specifically low-expressed in some HCCs. The unknown cDNA fragments LC2 7 and human Nip3 were obtained. The 99.4% homologous cDNA fragment of gene LC2 0 is highly expressed in some HCCs. Conclusion: Four HCC-related genes were identified. Among them, L2 and human aldolase B gene may be genes that inhibit the occurrence and development of HCC, while human Nip3 gene and LC2 7 may be genes that promote the occurrence and development of HCC. L2 and LC2 7 are new genes that are currently unknown.