目的建立注射用洋参生脉粉针剂中10种主要单体皂苷的含量测定方法。方法采用HPLC法,使用蒸发激光散射检测器,色谱柱为Kromasil NH2柱,乙腈水异丙醇进行三元梯度洗脱,分析测定注射用洋参生脉中各单体皂苷含量。结果供试品中主要单体皂苷与其他相关峰均能达到基线分离,且线性良好,回收率为96.9%~100.3%,RSD为1.0%~1.9%,r=0.999 2~0.999 9。结论该法是一种分析测定制剂中西洋参皂苷类成分的有效方法。 Objective To establish a method for the determination of ten main monomer saponins in injection powder for ginseng injection. Methods The HPLC method was used. Evaporative laser light scattering detector was used. The column was Kromasil NH2 column. Acetonitrile-water isopropanol was used to carry out the three-way gradient elution. The content of each monomer saponin in the ginseng pulse for injection was determined. Results The main monomer saponins could reach the baseline separation with other relevant peaks, and the linearity was good. The recovery was 96.9%~100.3%, RSD was 1.0%~1.9%, and r=0.999 2~0.999 9. Conclusion This method is an effective method for the analysis and determination of ginseng saponins in preparations.