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[目的]研究电压门控质子通道蛋白Hv1在结肠癌组织中的表达及其临床病理学意义。[方法]回顾性分析2004年5月至2008年5月的87例结肠癌患者临床病理学资料;免疫组化检测87例结肠癌组织和30例癌旁正常组织石蜡标本中Hv1表达。Cox风险回归模型回归分析影响结肠癌患者预后的独立影响因素。[结果]Hv1在结肠癌组织中呈阳性表达,在癌旁正常组织中呈阴性表达。Hv1表达水平与患者年龄、肿瘤大小、肿瘤T分期、淋巴转移、远处转移及临床分期等临床病理因素之间有显著性相关(P<0.05)。Kaplan-Meier生存分析显示,高表达Hv1的结肠癌患者预后差,与Hv1低表达的患者相比,Hv1高表达的患者中位生存期明显缩短(46个月vs.53个月,P<0.05),无复发中位生存时间也明显缩短(36个月vs.49个月,P<0.05)。COX回归分析显示Hv1表达是结肠癌患者总生存率(P=0.014)和无复发生存率(P=0.037)的独立影响因素。[结论 ]电压门控质子通道蛋白Hv1在结肠癌组织中阳性表达,是结肠癌患者预后生存的独立影响因素,是治疗结肠癌药物研发的潜在靶点。
[Objective] To investigate the expression of voltage-gated proton channel protein Hv1 in colon cancer and its clinicopathological significance. [Methods] The clinicopathological data of 87 patients with colon cancer from May 2004 to May 2008 were retrospectively analyzed. The expression of Hv1 was detected by immunohistochemistry in 87 specimens of colon cancer and 30 samples of adjacent normal tissues. Cox risk regression model regression analysis of prognostic factors in colon cancer patients independently. [Results] Hv1 was positive in colon cancer tissues and negative in adjacent normal tissues. There was a significant correlation between the expression level of Hv1 and clinicopathological factors such as age, tumor size, tumor T stage, lymph node metastasis, distant metastasis and clinical stage (P <0.05). Kaplan-Meier survival analysis showed that the prognosis of colon cancer patients with Hv1 overexpression was poor, and the median survival was significantly shorter in Hv1-overexpressing patients compared with patients with Hv1 overexpression (46 months vs.53 months, P <0.05 ), And the median recurrence-free survival was significantly shorter (36 months vs 49 months, P <0.05). COX regression analysis showed that Hv1 expression was an independent factor of overall survival (P = 0.014) and recurrence-free survival (P = 0.037) in patients with colon cancer. [Conclusion] The positive expression of voltage-gated proton channel protein Hv1 in colon cancer tissue is an independent influencing factor for the prognosis of colon cancer patients. It is a potential target for the development of drugs for colon cancer.