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目的探讨过氯酸铵(AP)对大鼠肺组织转化生长因子-β1(TGF-β1)和肿瘤坏死因子-α(TNF-α)mRNA表达的影响。方法将100只SD大鼠随机分为5组:48、96和192 mg／kg AP染毒剂量组、阴性对照组(生理盐水)和博莱霉素组(BLM5,mg/kg),采用一次性气管内注入染毒,染毒后第3、7、14和28天每组各处死5只大鼠,通过RT-PCR法测定肺组织中TGF-β1和TNF-αmRNA的表达。结果肺组织TGF-β1 mRNA表达:第3天3个AP染毒剂量组(1．73、1．38、1．64),第7、14天192 mg／kg剂量组(1．31、0．70)以及第28天96、192 mg/kg剂量组(1．25、2．25)均比阴性对照组增加,差异均有统计学意义(P<0．05或P<0．01);阴性对照组、BLM组和48 mg/kg量组随染毒后时间延长TGF-β1 mRNA表达均明显降低,而第28天96、192 mg/kg剂量组表达仍较高。肺组织中TNF-αmRNA表达:第3天96、192 mg/kg剂量组(1．01、1．13)、第7和14天192mg/kg剂量组(0．74、0．91)、比阴性对照组增加,差异均有统计学意义(P<0．05或P<0．01);各剂量组TNF-αmRNA表达在第3天最高,而后逐渐下降至正常水平。结论AP可使大鼠肺组织致纤维化细胞因子TGF-β1和TNF-αmRNA表达增加。
Objective To investigate the effects of ammonium perchlorate (AP) on the expression of transforming growth factor-β1 (TGF-β1) and tumor necrosis factor-α (TNF-α) mRNA in rat lungs. Methods 100 SD rats were randomly divided into 5 groups: 48, 96 and 192 mg / kg AP exposure dose groups, negative control group (saline) and bleomycin group (BLM5, mg / kg) After intratracheal instillation, 5 rats in each group were killed on the 3rd, 7th, 14th and 28th day after exposure. The expression of TGF-β1 and TNF-αmRNA in the lung tissue were determined by RT-PCR. Results The expression of TGF-β1 mRNA in lung tissue was significantly higher in the three AP-exposed groups (1.73,1.38 and 1.64) on the third day and 192 mg / kg on the seventh and 14th days .70) and the 96,192 mg / kg dose group (1.25,2.25) on day 28 were significantly higher than those in the negative control group (P <0.05 or P <0.01) ; Negative control group, BLM group and 48 mg / kg dose group with prolonged exposure time significantly decreased TGF-β1 mRNA expression, and the 28th day 96,192 mg / kg dose group was still high. TNF-αmRNA expression in lung tissue: 96,192 mg / kg dose group (1.01,1.13) on day 3, 192mg / kg dose group (0.74,0.91) on days 7 and 14, Negative control group increased, the difference was statistically significant (P <0.05 or P <0.01); TNF-αmRNA expression in each dose group the highest on the third day, and then gradually decreased to normal levels. Conclusion AP can increase the expression of fibroblast-derived cytokines TGF-β1 and TNF-αmRNA in rat lung tissue.