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目的:了解有氧运动对肺动脉高压大鼠肺动脉血管舒张反应的影响。方法:8周大的雄性SD大鼠,被随机分为4组,分别是常氧不运动组(N)、常氧运动组(EX)、肺动脉高压不运动组(H)、肺动脉高压运动组(HE)。将大鼠每天暴露在氧浓度为15.6～16.1%的环境中8小时,每周7次制作肺动脉高压模型。运动组分别进行8周,每天1小时,5天/周游泳训练,第9周分离出大鼠肺动脉,检测4组动脉环乙酰胆碱(Ach)诱导的内皮依赖性和硝普钠(SNP)诱导的非内皮依赖性舒张反应。结果:肺动脉高压组大鼠的肺动脉环的内皮依赖性血管舒张功能明显下降,动脉环经NO途径的最大舒张反应明显降低,有氧运动对肺动脉高压大鼠肺动脉的舒张功能有一定的改善作用,与肺动脉高压对照组及常氧运动组比较差异有统计学意义。结论:实验结果证实有氧运动对NO介导的血管舒张功能的有益调节作用,部分被低氧效应所抵消。长期低氧效应超过有氧运动对肺动脉血管的作用这个现象,很可能是与NO途径受损有关。
Objective: To understand the effects of aerobic exercise on pulmonary vasodilation in rats with pulmonary hypertension. Methods: Eight-week-old male Sprague-Dawley rats were randomly divided into 4 groups: normoxia non-exercise group (n), normoxia exercise group (EX), pulmonary hypertension non-exercise group (H), pulmonary hypertension group (HE). The rats were exposed daily for 15 hours to 16.1% oxygen concentration for 8 hours and 7 times a week to make pulmonary hypertension model. The rats in exercise group were trained on swimming for 8 weeks, 1 hour per day and 5 days / week. Pulmonary artery was isolated in the 9th week. Ach group induced endothelium-dependent and sodium nitroprusside (SNP) -induced Non-endothelium-dependent relaxation. Results: The endothelium-dependent vasodilatation of pulmonary artery rings was significantly decreased in pulmonary hypertensive rats, and the maximal vasodilatation of arterial rings via NO pathway was significantly reduced. Aerobic exercise improved the pulmonary artery diastolic function in pulmonary hypertensive rats, Compared with pulmonary hypertension control group and normoxic group, the difference was statistically significant. CONCLUSIONS: The experimental results confirm beneficial effects of aerobic exercise on NO-mediated vasodilation and are partly offset by hypoxia. Long-term hypoxic effects over aerobic exercise on the pulmonary artery of this phenomenon is likely to be impaired with the NO pathway.