植物组织培养的理论依据是植物细胞具有全能性,即植物的每一个细胞都具有母体的全部遗传信息。本文着重介绍了烟草、胡萝卜和月季的组织培养的具体操作过程,包括培养基配制、外植体的消毒、接种、培养、转移等步骤。不同植物组织的培养,需用不同的培养基,培养基中的激素种类及其用量也应不同,才能获得再生植株。 The rationale for plant tissue culture is that plant cells are pluripotent, that is, every cell in a plant has all the genetic information of the mother. This paper focuses on the specific operation of tissue culture of tobacco, carrot and rose, including preparation of medium, disinfection of explants, inoculation, culture, transfer and other steps. Different plant tissue culture, the need for different media, hormone types in the medium and its dosage should be different in order to obtain regenerated plants.