目的　建立高效液相色谱荧光法测定加替沙星注射液的含量方法。方法　CLC ODS柱 (15mm× 6.0mm ,5 μm ) ,流动相 :0 .0 5mol/L枸橼酸 乙腈 (80∶2 0 ) ,三乙胺调 pH3 .0 ,流速 1mL·min-1,Ex =3 60nm ,Em =465nm ,样品稀释 10 0 0倍 ,进样10 μL。 结果　加替沙星在 4～ 3 2ng范围内线性关系良好 ,回收率 (99.98± 0 .0 6) % ,日内、日间RSD <2 %。 结论　该法简便、快速、准确 ,可用于测定加替沙星注射液的含量。 Objective To establish a HPLC method for the determination of gatifloxacin injection. Methods CLC ODS column (15 mm × 6.0 mm, 5 μm) was used. The mobile phase consisted of 0.05 mol / L citric acid (80:20), triethylamine adjusted to pH 3.0, flow rate 1 mL · min- = 3 60nm, Em = 465nm, the sample is diluted 100 times and 10 μL is injected. Results Gatifloxacin showed a good linearity in the range of 4 ~ 32ng with a recovery of (99.98 ± 0.06)% and a daily RSD of less than 2%. Conclusion The method is simple, rapid and accurate and can be used to determine the content of gatifloxacin injection.