论文部分内容阅读
目的 研究苦参碱诱导K5 6 2细胞分化的信号转导机制。方法 以Fura 2 AM为荧光探针 ,定量分析苦参碱作用后K5 6 2细胞内钙离子浓度的动态变化。结果 经苦参碱处理后 ,在胞外有钙的情况下 ,K5 6 2细胞内钙离子浓度由 12 9.0 2nmol/L升至 2 0 7.5 0nmol/L ,并维持较长时间 ;在无胞外钙的情况下 ,胞内钙离子浓度由 12 9.0 2nmol/L迅速升至 16 4 .2 2nmol/L ,并迅速恢复。两者的增高幅度均与苦参碱浓度呈正相关。结论 在苦参碱诱导K5 6 2细胞分化过程中 ,钙离子浓度的变化是一重要的与细胞的增殖抑制和分化相关的早期信号
Objective To study the signal transduction mechanism of matrine-induced K562 cell differentiation. Methods Fura 2 AM was used as a fluorescent probe to quantitatively analyze the dynamic changes of intracellular Ca2 + concentration in K562 cells treated with matrine. Results After treatment with matrine, the intracellular Ca2 + concentration in K562 cells increased from 12 9.0 2 nmol / L to 20 07 0 nmol / L and maintained for a long time in the presence of extracellular calcium. In the absence of extracellular In the case of calcium, the intracellular calcium concentration rose rapidly from 12 9.0 2 nmol / L to 16 4 .2 2 nmol / L and quickly recovered. The increase of both was positively correlated with matrine concentration. Conclusion In the process of matrine-induced K562 cell differentiation, the change of calcium concentration is an important early signal correlated with the inhibition of proliferation and differentiation of cells