论文部分内容阅读
为探讨体外人牙髓细胞的生物学特性，采用体外细胞连续培养、钙质染色、Ｘ射线能谱分析与透射电镜观察等方法，对体外人恒牙牙髓细胞的矿化特性进行了研究并与人牙龈成纤维细胞（ｈｕｍａｎｇｉｎｇｉｖａｆｉｂｒｏｂｌａｓｔｓ，ＨＧＦｓ）进行了比较。结果表明，人牙髓细胞在体外可以复层生长并形成细胞结节，ＨＧＦｓ则无此能力，牙髓细胞的碱性磷酸酶活性亦较ＨＧＦｓ者高；细胞结节钙质染色呈阳性，结节内钙磷含量明显增高；人牙髓细胞有许多与成牙本质细胞相似的超微结构特征，胞间基质中可见致密晶状小体。结果提示，体外连续培养的人牙髓细胞有向成牙本质细胞分化的可能，这可为研究人牙髓细胞的分化和矿化提供有价值的思路。
In order to investigate the biological characteristics of human dental pulp cells in vitro, the mineralization characteristics of human dental pulp cells in vitro were studied by continuous cell culture, calcium staining, X-ray energy dispersive spectroscopy and transmission electron microscopy. Human gingival fibroblasts (humangingivafibroblasts, HGFs) were compared. The results showed that human dental pulp cells could be stratified in vitro and formed nodules, but HGFs did not have the ability and alkaline phosphatase activity of dental pulp cells was higher than that of HGFs. Cell nodules were positive for calcium staining, The content of calcium and phosphorus in the section was significantly increased. There were many ultrastructural features in human dental pulp cells that were similar to odontoblasts, and dense crystalline bodies were observed in the interstitial matrix. The results suggest that human dental pulp cells cultured in vitro have the potential to differentiate into odontoblasts, which can provide valuable ideas for studying the differentiation and mineralization of human dental pulp cells.