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目的探讨mi R-423、mi R-205和mi R-1307单核苷酸多态性与宫颈癌的相关性。方法以病例-对照研究的方法收集104例宫颈癌及186例骨折、高血压等同期非肿瘤患者为对照,采用PCR-限制性酶切片段长度多态性技术对mi R-423 rs6505162位点进行基因型分析;采用PCR和多重连接酶检测反应技术对mi R-205 rs3842530和mi R-1307 rs7911488基因多态性进行分析。结果 rs6505162 CC、rs6505162 AC和rs6505162AA基因型在宫颈癌组中分布为64.4%、31.7%和3.8%,对照组分布为66.1%、30.1%和3.8%;rs3842530-/-、-/insert和inser/inser基因型在宫颈癌组中分布为56.7%、34.6%和8.7%,对照组分布为52.7%、36.0%和7.0%;rs7911488 AA、AG和GG基因型在宫颈癌组中分布为51.9%、39.4%和8.7%,对照组分布为45.1%、45.1%和9.7%。各位点基因型分布比较,差异均无统计学意义(P>0.05)。进一步按年龄分层分析显示,≤50岁和>50岁不同年龄组各基因型分布在对照组和宫颈癌组中比较,差异无统计学意义(P>0.05)。结论 mi R-423 rs6505162、mi R-205 rs3842530和mi R-1307 rs7911488基因多态性可能与宫颈癌发生无关。
Objective To investigate the association of mi R-423, mi R-205 and mi R-1307 single nucleotide polymorphisms with cervical cancer. Methods A total of 104 cases of cervical cancer and 186 cases of non-tumor patients with fracture and hypertension were collected as case-control study. The mi R-423 rs6505162 site was detected by PCR-restriction fragment length polymorphism Genotype analysis. The polymorphisms of mi R-205 rs3842530 and mi R-1307 rs7911488 were analyzed by PCR and multiplex ligase assay. Results The rs6505162 CC, rs6505162 AC and rs6505162AA genotypes were 64.4%, 31.7% and 3.8% in the cervical cancer group and 66.1%, 30.1% and 3.8% in the control group. Rs3842530 - / -, - / insert and inser / The inser genotypes were 56.7%, 34.6% and 8.7% in the cervical cancer group and 52.7%, 36.0% and 7.0% in the control group. The genotypes of rs7911488 AA, AG and GG in the cervical cancer group were 51.9% 39.4% and 8.7% in the control group, and 45.1%, 45.1% and 9.7% in the control group. There was no significant difference in the distribution of genotypes between sites (P> 0.05). Further analysis by age stratification showed that there was no significant difference in the distribution of genotypes in different age groups ≤50 years and> 50 years old in the control group and cervical cancer group (P> 0.05). Conclusion The polymorphisms of mi R-423 rs6505162, mi R-205 rs3842530 and mi R-1307 rs7911488 may not be related to the occurrence of cervical cancer.