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为了深入探讨远离大脑局部致伤后ＣＮＳ总体应变效应，阐明其与神经─体液ＥＴ１启动、调控的作用与关系，本研究用放免法和斑点杂交监测了犬双后肢低、高速投射物致伤后血浆、ＣＳＦ、海马、下丘脑和颞叶灰、白质ＥＴ１含量动态变化。结果：高、低速组伤后血浆、ＣＳＦ、各脑区ＥＴ１含量和ＥＴ１─ｍＲＮＡ表达水平均显著高于伤前和对照组。血浆、ＣＳＦ、各脑区ＥＴ值相互间均呈显著正相关关系。提示：远离大脑局部致伤后神经─体液ＥＴ１含量变化有其自身规律和内在联系，在ＣＮＳ应变效应的发生、发展病理生理过程中可能起持续而重要的作用。
In order to further explore the overall strain effect of CNS after local injury to the brain and to elucidate its role in the initiation and regulation of neuro-humoral ET1, we examined the effects of low and high-speed projectile hindlimbs by radioimmunoassay and dot blotting Plasma, CSF, hippocampus, hypothalamus and temporal lobe gray matter, white matter ET1 content dynamic change. Results: The content of ET1 and the expression of ET1-mRNA in plasma, CSF and brain regions of high and low speed group were significantly higher than those of pre-injury group and control group. There were significant positive correlations between plasma ET, ET and ET in various brain regions. It is suggested that ET1 content in neuro-humoral fluid may play a continuous and important role in the occurrence of CNS strain and the development of pathophysiological processes.