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目的建立同时测定救必应药材中紫丁香苷、丁香脂素4’-O-β-D-吡喃葡萄糖苷、长梗冬青苷、铁冬青酸含量的方法。方法采用Kromasil 100-5 C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈(A)-0.02%三氟乙酸水溶液(B),梯度洗脱,流速为1.0 m L·min-1,检测波长为210 nm,柱温为30℃。结果 4种成分均达到基线分离,线性关系良好(r≥0.9999);平均加样回收率为98.16%~101.3%,RSD<3.0%(n=6)。结论该方法简单准确可靠,可为救必应药材的质量控制提供科学依据。
OBJECTIVE To establish a method for the simultaneous determination of syringin, syringalid 4’-O-β-D-glucopyranoside, long stem glycosides and iron-tungstic acid in QiBing medicinal materials. Methods A Kromasil 100-5 C18 column (250 mm × 4.6 mm, 5 μm) was used with a mobile phase of acetonitrile (A) - 0.02% trifluoroacetic acid (B) and gradient elution at a flow rate of 1.0 mL · min -1 , The detection wavelength was 210 nm, the column temperature was 30 ℃. Results The four components all achieved baseline separation with good linearity (r≥0.9999). The average recovery was 98.16% ~ 101.3% with RSD <3.0% (n = 6). Conclusion The method is simple, accurate and reliable, which can provide a scientific basis for the quality control of Chinese herbal medicines.