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以高需冷量葡萄品种‘夏黑’为试材,石开究短日照、长日照和自然光照3个条件下,葡萄冬芽休眠的自然诱导因子及其对休眠诱导期冬芽呼吸代谢的调控机制.结果表明:自然低温、短日照2个环境因素单独或共同作用均能诱导葡萄冬芽进行自然休眠.短日照在诱导葡萄冬芽进入自然休眠的过程中起主导作用启然低温起辅助作用;温度相同条件下,日照时间越短对葡萄冬芽自然休眠的诱导作用越强.总呼吸速率达到峰值是葡萄冬芽休眠诱导期结束的标志.在自然休眠诱导期间,葡萄冬芽磷酸戌糖途径运行活性和容量占总呼吸的比例迅速上升,其中自然条件的葡萄冬芽分别由16.0%和20.1%上升至22.3%和26.0%.自然低温是诱导葡萄冬芽底物氧化水平上呼吸途径发生变化的主导因素,短日照起促进作用.在葡萄冬芽自然休眠诱导期间,交替途径运行活性和容量占总呼吸的比例迅速上升,其中自然条件葡萄冬芽分别由19.4%和27.3%上升至38.2%和46.8%.自然低温和短日照均可诱导葡萄冬芽电子传递链水平上呼吸途径发生变化.
Taking the high-yielding grape variety Xiaya as test material, the natural inducing factors of dormancy in winter grape buds and their regulation on the respiratory metabolism of winter buds during dormancy induction were studied under three conditions: short-day, long-day and natural daylight The results showed that natural low-temperature and short-day two environmental factors alone or in combination can induce the natural dormancy of winter grape bud.Dark sunshine played a leading role in inducing the winter grape bud into the natural dormancy, The shorter the sunshine duration was, the stronger the induction effect was on the natural dormancy of winter grape buds.The peak of total respiration rate was the indicator of the end of dormancy induction in winter grapevine.During the natural dormancy induction, the activity and capacity The proportion of total respiration increased rapidly, of which the natural conditions of winter grape buds increased from 16.0% and 20.1% to 22.3% and 26.0%, respectively. Natural hypothermia was the dominant factor that induced the changes of respiratory pathway in the oxidative level of winter grape buds. Promote the role.In the grape winter bud during the natural dormancy induction, the alternating route of activity and capacity of the proportion of total respiration rapidly Wherein vine bud natural conditions rose from 19.4%, and 27.3 to 38.2% and 46.8%. NATURAL low temperature and short days can induce changes in the respiratory route vine bud electron transport chain level.