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目的研究纳米二氧化钛(TiO2)对大鼠的氧化应激损伤效应,以及蜂胶对这种损伤的防护效果。方法采用无特定病原体级雄性SD大鼠35只,随机分为7组,每组5只。低、中、高剂量染毒组分别灌服给药剂量为8、16、32 mg/(kg体质量·d)纳米TiO2溶液,共7 d;低、中、高剂量蜂胶干预组于灌服0.2 g/(kg体质量·d)蜂胶后,间隔4 h,再分别灌服8、16、32 mg/(kg体质量·d)纳米TiO2溶液,灌服7 d后,中、高剂量蜂胶干预组分别继续灌服0.2g/(kg体质量·d)蜂胶7和14 d;对照组大鼠仅灌服灭菌生理氯化钠溶液2 ml,共7 d。采集各组大鼠下腔静脉血,分离血清,采用分光光度法测定血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)水平。结果与对照组比较,中、高剂量染毒组SOD活力下降[(1 187.0±5.7)vs(1 215.2±21.7)×103μmol/(min·L),(1 154.4±10.4)vs(1 215.2±21.7)×103μmol/(min·L),P<0.05],MDA水平增加[(1.71±0.03)vs(1.62±0.07)μmol/L,(1.78±0.03)vs(1.62±0.07)μmol/L,P<0.05]。大鼠血清中SOD活力随着纳米TiO2剂量的增加而降低[回归方程:^y=-1.820 x+1 213.000,决定系数(R2)=0.748,P<0.01],MDA水平随着纳米TiO2剂量的增加而增加[回归方程:^y=0.005 x+1.630,R2=0.656,P<0.01]。蜂胶干预处理大鼠的SOD活力高于染毒处理大鼠(P<0.01);蜂胶干预处理大鼠的MDA水平低于染毒处理大鼠(P<0.01)。结论在本实验条件下,纳米TiO2可引起大鼠的氧化损伤,蜂胶能够增强大鼠的抗氧化应激能力。
Objective To study the oxidative stress damage effect of nano-TiO2 on rats and the protective effect of propolis on this damage. Methods Thirty-five male Sprague Dawley rats without specific pathogen were randomly divided into 7 groups with 5 rats in each group. Low, medium and high dose exposure groups were dosed with 8,16,32 mg / (kg body weight · d) of nano-TiO2 solution for 7 days respectively. 0.2 g / (kg body weight · d) propolis, and then every 4 h intervals, then dosed with 8,16,32 mg / (kg body weight · d) of nano-TiO2 solution, In the intervention group, 0.2g / (kg body weight · d) propolis was continuously administered for 7 and 14 days respectively. In the control group, only 2 ml sterile physiological sodium chloride solution was administered for 7 days. The inferior vena cava blood of each group was collected and the serum was separated. The activity of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured by spectrophotometry. Results Compared with the control group, the activities of SOD in the medium and high dose groups were significantly lower than those in the control group ([(187 ± 5.7) vs (115.2 ± 21.7) × 103μmol / (min · L), (1554.4 ± 10.4) vs (1.71 ± 0.03) vs (1.62 ± 0.07) μmol / L, (1.78 ± 0.03) vs (1.62 ± 0.07) μmol / L respectively, P <0.05) P <0.05]. The activity of SOD in the serum of the rats decreased with the increase of the dosage of nano-TiO2 [Regression equation: y = -1.820 x + 1 213.000, the determination coefficient (R2) = 0.748, P <0.01] Increase and increase [regression equation: ^ y = 0.005 x + 1.630, R2 = 0.656, P <0.01]. The activity of SOD of the rats treated with propolis was higher than that of the rats treated with propolis (P <0.01). The MDA level of rats treated with propolis was lower than that of the rats treated with propolis (P <0.01). Conclusion Under the experimental conditions, nano-TiO2 can cause oxidative damage in rats, propolis can enhance antioxidant capacity of rats.