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目的:研究Dawson结构钨磷酸钾盐化合物(K6[P2W18O62]·14H2O)的体外抗肿瘤活性。方法:取人乳腺癌细胞MCF-7加入不同浓度K6[P2W18O62]·14H2O处理,应用MTT法分析细胞增殖计算半数抑制浓度(IC50);用流式细胞术分析细胞周期和细胞凋亡,计算各期细胞比例及细胞凋亡率;用台盼蓝染色和细胞计数方法分析细胞生长与细胞活力计算活细胞百分率;与表阿霉素合用研究其协同抗肿瘤作用。结果:K6[P2W18O62]·14H2O对MCF-7细胞的增殖抑制的IC50值为(33.7±3.2)μmol·L-1,其作用于细胞后可降低S期和G1期细胞比例,48h诱导细胞凋亡率为3.7%～29.2%,72h活细胞百分率为95.37%～76.78%,与表阿霉素合用后细胞增殖抑制率显著高于后者单用(P<0.05)。结论:K6[P2W18O62]·14H2O能显著抑制MCF-7细胞增殖并诱导细胞凋亡,并能增强表阿霉素的抗肿瘤作用。
AIM: To study the antitumor activity of Dawson structure potassium tungstophosphate (K6 [P2W18O62] · 14H2O) in vitro. Methods: Human breast cancer cell line MCF-7 was treated with different concentrations of K6 [P2W18O62] · 14H2O. The half inhibitory concentration (IC50) was calculated by MTT assay. Cell cycle and apoptosis were analyzed by flow cytometry. Cell ratio and apoptosis rate; assay cell growth and cell viability by trypan blue staining and cell counting method to calculate the percentage of viable cells; with epirubicin in combination to study its synergistic anti-tumor effect. Results: The IC50 value of K6 [P2W18O62] · 14H2O on proliferation inhibition of MCF-7 cells was (33.7 ± 3.2) μmol·L-1, which decreased the proportion of cells in S phase and G1 phase after 48 h The mortality rate was 3.7% -29.2%, the percentage of viable cells at 72h was 95.37% -76.78%, and the cell proliferation inhibition rate was significantly higher than that of epirubicin alone (P <0.05). Conclusion: K6 [P2W18O62] · 14H2O can significantly inhibit MCF-7 cell proliferation and induce apoptosis, and can enhance the antitumor effect of epirubicin.