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目的 探讨铝的发育毒性及机理。方法 孕9.5d SD大鼠胚胎于体外培养系统中给予不同剂量（以Al3+计,0.6、0.9、1.2、3.0、6.0和9.0μg/ml）的 AlCl3,培养48h后,对胚胎进行Bgown’s形态评分,测量卵黄囊直径、胚胎体长及头长、胚胎干重。结果 随着铝剂量的增加,反映胚胎生长发育和器官分化的各项指标呈现出下降趋势,有一定的剂量-效应关系（r=-0.890～-0.973,P<0.001）。其中,卵黄囊及血管网发育分化、心脏发育及体翻转在Al3+剂量为1.2μg/ml时与对照组比较下降有显著性（P<0.05）。Al3+浓度大于或等于3.0μg/ml,胚胎发育及形态分化显著受到抑制（P<0.05或P<0.01）,同时胚胎畸形发生率明显升高（P<0.05）,主要表现为神经管闭合不全、脑发育不良和体翻转不全。结论AlCl3有一定的胚胎毒性和致畸性。
Objective To investigate the developmental toxicity and mechanism of aluminum. Methods Different doses of AlCl3 (0.6, 0.9, 1.2, 3.0, 6.0 and 9.0 μg / ml, respectively) were given to the embryos of pregnant SD rats in vitro for 9.5 days. After culturing for 48 hours, Bgown’s morphological score, Yolk sac diameter measurement, body length and head length, embryo dry weight. Results As the dose of aluminum increased, the indexes reflecting embryonic growth and organ differentiation showed a downward trend with a dose-response relationship (r = -0.890-0.973, P <0.001). Among them, the development and differentiation of yolk sac and vascular network, heart development and body turnover were significantly decreased compared with the control group (P <0.05) when Al3 + dosage was 1.2μg / ml. Al3 + concentration was greater than or equal to 3.0μg / ml, embryonic development and morphological differentiation were significantly inhibited (P <0.05 or P <0.01), while embryo malformations significantly increased (P <0.05), mainly manifested as neural tube insufficiency, Brain dysplasia and body turnover incomplete. Conclusion AlCl3 has some embryotoxicity and teratogenicity.