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采用高效液相色谱法,系统研究了不同降解菌及其多元混配在土壤中对氯嘧磺隆的降解作用。结果表明:真菌黑曲霉、青霉、F8和F31酿酒酵母在土壤中对氯嘧磺隆的降解速度存在显著差异,而且均能加快氯嘧磺隆的降解速度,其降解能力黑曲霉>F31>青霉>F8。黑曲霉分别和青霉、F8、F31混合后,各处理间差异显著,并且随着黑曲霉比例的增加,对土壤中除草剂的降解能力明显增强,其中F31和黑曲霉混合后效果最佳,降解率可达86.43%。对降解菌进行三元混配后发现,在二元复合的基础上添加少量青霉可进一步提高降解能力。
High performance liquid chromatography (HPLC) was used to systematically study the degradation of chlorimuron-ethyl by different degradative bacteria and their multiple mixture in the soil. The results showed that fungi Aspergillus niger, Penicillium, F8 and F31 Saccharomyces cerevisiae had significant differences in the degradation rate of chlorimuron-ethyl in soil, and both could accelerate the degradation rate of chlorimuron-ethyl. The degrading ability of Aspergillus niger F31> Penicillium> F8. Aspergillus niger and Penicillium, F8, F31 mixed, the significant difference between the various treatments, and as the proportion of Aspergillus niger increased degradation of soil herbicides significantly enhanced, of which F31 and Aspergillus niger mixed the best, Degradation rate up to 86.43%. After three-way compounding of the degrading bacteria, it was found that adding a small amount of Penicillium on the basis of binary compound can further improve the degradation ability.