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目的 探讨利多卡因对短暂脑缺血后海马区迟发性神经元降解的影响。方法 脑缺血模型为兔脑四血管夹闭模型 ,2 5只家兔随机分成三组 :假手术组 (Sh ,n =5 ) ;缺血组 (Is,n =10 ) ,夹闭双侧颈总动脉和椎动脉 5min后恢复脑灌注 ;利多卡因组 (Li,n =10 ) ,夹闭颈总和椎动脉前 5min给予利多卡因 10mg/kg。 3组均于 3d后取脑行病理HE染色和TUNEL染色 ,对海马区阳性细胞进行计数。结果 HE染色发生缺血改变细胞数和TUNEL染色细胞凋亡数有显著相关性 (r =0 915 ) ;均显示Is和Li组比Sh组细胞凋亡数显著增加 (P <0 0 5 ) ,Li组比Is组细胞凋亡数显著增加 (P <0 0 5 )。结论 利多卡因加重短暂脑缺血后海马区的细胞凋亡
Objective To investigate the effect of lidocaine on delayed neuron degeneration in hippocampus after transient cerebral ischemia. Methods The model of cerebral ischemia in rabbits was occluded by four vessels in the brain. Twenty-five rabbits were randomly divided into three groups: sham operation group (Sh, n = 5), ischemic group (Is, n = 10) The carotid artery and vertebral artery were recovered 5min after cerebral perfusion; lidocaine group (Li, n = 10), lidocaine 10mg / kg 5min before clamping neck and vertebral artery. Three groups were taken after 3 days brain pathological HE staining and TUNEL staining, positive cells in the hippocampus were counted. Results There was a significant correlation between the number of apoptotic cells and the number of TUNEL-stained cells in HE stain (r = 0 915), which showed that the number of apoptotic cells in Is and Li groups was significantly higher than that in Sh group (P <0.05) The number of apoptotic cells in Li group was significantly higher than that in Is group (P <0.05). Conclusion Lidocaine aggravates apoptosis in hippocampus after transient cerebral ischemia