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目的:评价近年上市的肝水解肽生物学活性质量及其活性测定方法的应用。方法:应用正常人肝L02细骨包/WST-8比色法测定不同厂家的肝水解肽生物学活性,比较各个厂家产品质量以及评价该生物学活性测定法的应用情况。结果:共测定了10个厂家43批肝水解肽生物学活性,其中35批产品具有促进肝细胞生长的生物学活性,4个厂家的8批产品无促进肝细胞生长的生物学活性。不合格产品中有1个厂家的2批产品已过效期,3个厂家的6批产品均在效期内。结论:该法可用于肝水解肽生物学活性的测定,是否在有效期内对样品的活性无明显影响。
OBJECTIVE: To evaluate the biological activity of hepatic hydrolyzate marketed in recent years and the application of the assay. Methods: The biological activity of hepatic hydrolyzing peptides from different manufacturers was determined by normal liver L02 / BST / WST-8 colorimetric method. The product quality of each manufacturer was compared and the application of this biological activity assay was evaluated. Results: A total of 43 batches of hepatic hydrolytic peptides were assayed in 10 batches. Among them, 35 batches of batches of batches had the biological activity of promoting hepatocyte growth and 8 batches of batches of 4 batches had no biological activity to promote hepatocyte growth. 2 products of 1 manufacturer failed in expired products have expired, 6 products of 3 manufacturers are in expiration date. Conclusion: This method can be used to determine the biological activity of hepatic hydrolyzate, and has no significant effect on the activity of the sample during the expiration date.