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无精子症占不育男性的10%~15%[1],是男性不育中最为严重的一种,病因复杂。无精子症患者可通过经皮附睾穿刺(PESA)结合卵细胞胞质内单精子注射(ICSI)达到生育目的。PESA操作简便、患者痛苦少,获得精子的概率为85%[2],并有较高的精子活动率[3],且受精率、卵裂率、妊娠率等也较高[4]。ICSI使用的精子必须是活精子,然而,大约15%的附睾穿刺液标本,其全部精子均是d级精子[2]。因为不活动的精子并不意味着是死精子[5],此时评估精子的存活率就非常必要。而相比精液精
Azoospermia accounts for 10% to 15% of infertile men [1], is one of the most serious male infertility, the etiology is complicated. Patients with azoospermia can achieve fertility through percutaneous epididymal puncture (PESA) combined with intracytoplasmic sperm injection (ICSI). PESA is simple, less painful and has a sperm motility of 85% [2], high sperm motility [3] and high rates of fertilization, cleavage and pregnancy [4]. The sperm used by ICSI must be live sperm, however, about 15% of epididymal puncture fluid specimens, all of which sperm are d grade sperm [2]. Because inactive sperm does not mean dead sperm [5], assessing sperm survival is necessary. And compared to seminal essence