论文部分内容阅读
The single tryptophan residue of trichosanthin was cleaved by the use of BNPS-skatolereaction with STIG2 peptide fregment which was obtained from the tryptic digestion of N-succinyltrichosanthin.After separation,the N-terminal sequence of tryptophanyl C-side peptide was deter-mined by use of manual DABITC-PITC double coupling sequencing method as follows:-Leu-Ala-Leu-Ser-Lys-Gln-Ile-Gln-Ile-Ala-.
The single tryptophan residue of trichosanthin was cleaved by the use of BNPS-skatole reaction with STIG2 peptide fregment which was obtained from the tryptic digestion of N-succinyltrichosanthin. After the separation, the N-terminal sequence of tryptophanyl C-side peptide was deter- mined by use of manual DABITC-PITC double coupling sequencing method as follows: -Leu-Ala-Leu-Ser-Lys-Gln-Ile-Gln-Ile-Ala-.