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选用两组转基因品质改良大豆(富含硫氨基酸),利用磷脂脂肪酸(PLFA)法分析了在大田种植条件下,两组转基因大豆对土壤微生物群落结构的影响。结果表明,与非转基因大豆相比,转基因品系根际土壤微生物群落的结构发生明显的变化。在两组转基因品系中PLFA组分均发生变化,A组中检测到15种特异性的PLFA,其中占主导地位的分别是是革兰氏阳性菌(GP)的特征脂肪酸16∶0、10Me18∶0和好氧细菌特征脂肪酸18∶1ω7c;B组中检测到13种特异性的PLFA,其中占主导地位的是表征GP的脂肪酸15∶0、16∶0和非特征脂肪酸14Me16∶0。在A组的OE-8品系及B组的17-4和57品系中,表征细菌的PLFA含量和总PLFA含量与非转基因品系相比有显著上升。结果表明种植转基因大豆影响根际土壤微生物群落结构。
Two groups of genetically modified soybeans (sulfur-rich amino acids) were selected. The effects of two groups of transgenic soybeans on soil microbial community structure were analyzed using PLFA method under field conditions. The results showed that compared with non-GMO soybean, the structure of rhizosphere soil microbial community in transgenic lines changed obviously. PLFA components changed in both transgenic lines, and 15 specific PLFAs were detected in group A, of which the dominant fatty acids were Gram-positive bacteria (GP), 16:0, 10 Me18: 0 and aerobic bacteria characteristic fatty acids 18: 1ω7c. Thirteen specific PLFAs were detected in group B, of which the dominant fatty acids were 15: 0, 16: 0 and GPR: non-characteristic fatty acids: 14Me16: 0. In group AEO-8 and group B, lines 17-4 and 57, the PLFA content and the total PLFA content of the bacteria were significantly increased as compared with the non-transgenic lines. The results showed that planting transgenic soybean affected rhizosphere soil microbial community structure.