一、取材、消毒和接种选切花型菊花品种“美粉”、“紫落金星”、“绿牡丹”的健壮无病毒植株。在开花期,从主茎或侧枝上剪取尚未开放的小花蕾(直径0.3—0.5厘米)。先用自来水冲洗,再经过常规消毒,在无菌室或超净工作台上无菌操作。用消过毒的滤纸吸干水分,将花蕾纵切开,剥下萼片、花瓣、花托,分别接种到固体培养基上。二、培养方法1.一步成苗培养:将外植体接到 MS 附加 BA3毫克升、NAA1毫克升培养基上。温度26—27℃,每日光照8—9小时,光照强度为 First, drawing, disinfection and inoculation cut flowers selected chrysanthemum varieties “beauty powder”, “Purple Falling Venus”, “Green Peony” robust virus-free plants. At flowering, small buds (0.3-0.5 cm in diameter) that have not yet been opened are cut from the main stem or side branches. Rinsing with tap water, followed by routine disinfection, aseptic handling in sterile rooms or clean benches. Use disinfectant filter paper to dry the water, the flower bud vertical cut, peel sepals, petals, receptacle, were inoculated into solid medium. Second, training methods 1. One-step seedling culture: The explants received MS attached BA3 mg liters, NAA1 mg liters of culture medium. Temperature 26-27 ℃, daily light 8-9 hours, light intensity