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研究建立准确的基于人白细胞抗原一DPB1(HLA-DPB1)核酸序列的基因分型技术,为疾病与HLA基因相关性研究及器官、组织移植提供准确的HLA基因分型方法。使用第十一届国际组织相容性会议提供的引物,经PCR技术扩增、分离相应的基因片段,纯化后用PE公司四色荧光染料终止剂标记循环测序技术直接测定核酸序列,获得个体基因型序列资料,通过与基同型资料数据库比较确定基因型别。这一技术可以准确地确定个体的 HLA-DPB1的基因型别并得到核酸序列。为进一步研究奠定了基础。本技术可用于其它类似的研究工作。
To establish an accurate genotyping technique based on the human leukocyte antigen-DPB1 (HLA-DPB1) nucleic acid sequence and to provide an accurate HLA genotyping method for the study of the relationship between the disease and HLA gene and organ and tissue transplantation. Using the primers provided by the 11th International Organizational Compatibility Conference, the corresponding gene fragments were amplified by PCR and purified, and then the nucleic acid sequences were directly determined by the PE company’s four-color fluorescent dye terminator labeling cycle sequencing technology to obtain individual genes Type sequence data to determine genotypes by comparison with base-type data databases. This technique can accurately determine the genotype of an individual’s HLA-DPB1 and obtain a nucleic acid sequence. Laid the foundation for further research. This technique can be used for other similar research.