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目的了解杭州地区人博卡病毒(HBoV)的流行情况和基因变异情况。方法对杭州地区160例儿童急性呼吸道感染患者咽拭子样本进行HBoV检测,并对阳性样本进行常见呼吸道病毒的检测,最终选取HBoV单一阳性的HZ1株和HZ2株进行VP1基因扩增,将获得的序列上传Genbank并与其他国家和地区的8株HBoV的VP1基因进行序列比对和分析。结果杭州地区HBoV的检出率为6.9%(11/160),与其他常见呼吸道病毒的合并感染率达到45.5%(5/11),HZ1株和HZ2株与其他国家和地区的8株HBoV的VP1基因相似性达到99%以上。结论研究证明杭州地区急性呼吸道感染患儿中存在HBoV的感染,与其他呼吸道病毒有较高的合并感染,从分离到的HZ1株和HZ2株来看,杭州地区流行的是ST2基因型,其外壳蛋白VP1基因变异很小。
Objective To understand the prevalence and genetic variation of human Bocavirus (HBoV) in Hangzhou area. Methods HBoV was detected in throat swab samples of 160 children with acute respiratory infection in Hangzhou and the common respiratory viruses were detected in the positive samples. Finally, VP1 gene was amplified by single positive HZ1 and HZ2 strains of HBoV. The sequences were uploaded into Genbank and sequenced and analyzed with the VP1 genes of eight HBoV strains from other countries. Results The detection rate of HBoV in Hangzhou was 6.9% (11/160) and the rate of co-infection with other common respiratory viruses was 45.5% (5/11). The prevalence of HBoV in HZ1 and HZ2 strains was significantly higher than that in other countries VP1 gene similarity of more than 99%. Conclusions The study showed that HBoV infection was found in children with acute respiratory infection in Hangzhou area, which was associated with higher respiratory infection. The HZ1 and HZ2 strains isolated from Hangzhou showed that the ST2 genotype was popular in Hangzhou. Protein VP1 gene variation is small.