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目的:观察嵌合型单克隆抗体CH12对头颈部鳞状细胞癌(head and neck squamous cell carcinomas,HNSCC)裸鼠种植瘤生长的抑制作用,为进一步研究CH12单抗在肿瘤治疗中的作用提供参考数据。方法:Western blotting检测5种HNSCC细胞系A253、CAL27、Detroit 562、FaDu和RPMI 2650中表皮生长因子受体(epidermal growth factor receptor,EGFR)的表达,流式细胞术检测CH12单抗同这5种细胞系的结合能力。皮下接种CAL27和A253细胞,建立HNSCC裸鼠种植瘤模型。模型鼠腹腔注射CH12单抗,以PBS作为阴性对照,观察肿瘤生长情况,绘制肿瘤生长曲线。结果:EGFR在CAL27、A253、FaDu及Detroit 562细胞中均有不同程度的表达,其中CAL27细胞中EGFR的表达水平最高,A253细胞次之。CH12单抗与5种HNSCC细胞系的结合能力由高到低依次为CAL27、FaDu、A253、Detroit 562和RPMI 265细胞。CH12单抗对CAL27和A253细胞裸鼠种植瘤的生长均有显著抑制作用,抑瘤率分别为56.8%(P=0.022)和59.7%(P=0.015)。结论:单克隆抗体CH12对EGFR高表达的HNSCC细胞种植瘤的生长具有明显的抑制作用。
OBJECTIVE: To observe the inhibitory effect of chimeric monoclonal antibody CH12 on the growth of implanted tumors in nude mice with head and neck squamous cell carcinomas (HNSCC), and to provide a reference for further study on the role of CH12 monoclonal antibody in tumor therapy data. Methods: The expressions of epidermal growth factor receptor (EGFR) in five HNSCC cell lines, A253, CAL27, Detroit 562, FaDu and RPMI 2650, were detected by Western blotting. Flow cytometry Cell line binding ability. CAL27 and A253 cells were inoculated subcutaneously, and the implanted tumor model of HNSCC was established. The model mice were intraperitoneally injected with CH12 monoclonal antibody, with PBS as a negative control to observe the tumor growth and draw the tumor growth curve. Results: The expression of EGFR in CAL27, A253, FaDu and Detroit 562 cells were all significantly different. The expression of EGFR in CAL27 cells was the highest, followed by A253 cells. The binding ability of CH12 monoclonal antibody to five HNSCC cell lines from high to low was CAL27, FaDu, A253, Detroit 562 and RPMI 265 cells. CH12 monoclonal antibody could significantly inhibit the growth of implanted tumors in CAL27 and A253 nude mice, and the tumor inhibition rates were 56.8% (P = 0.022) and 59.7% (P = 0.015), respectively. Conclusion: Monoclonal antibody CH12 can significantly inhibit the growth of implanted tumor of HNSCC cells with high expression of EGFR.