肝细胞癌变过程,除去在细胞形态和代谢活动方面发生变化外,而且还呈现出某些胚胎肝的表型,如甲胎蛋白和胚胎性同功酶谱等。这些胚胎性蛋白在肝癌细胞中的再现,可能是由于肝细胞基因表达的调控系统,在肝细胞癌变过程中发生了异常变化,以至使己分化成熟的成年肝细胞去分化,进而发展为恶变细胞。我们用二乙基亚硝胺(DENA)诱发大鼠肝癌,在肝癌发生过程中,观察基因调控失调,及其与肝癌发生的关系。首先, The hepatocellular carcinogenesis process, in addition to changes in cell morphology and metabolic activity, but also presents some of the embryonic liver phenotype, such as alpha fetoprotein and embryonic isoenzyme spectrum. The reproduction of these embryonic proteins in hepatoma cells may be due to the regulation system of hepatocyte gene expression. Abnormal changes occur during the hepatocellular carcinogenesis, so that differentiated and mature adult hepatocytes are dedifferentiated, and then become malignant cells. . We used diethylnitrosamine (DENA) to induce rat liver cancer. During the development of liver cancer, we observed dysregulation of gene regulation and its relationship with liver cancer. First of all,