在超声波法辅助提取的基础上,采用L9(34)正交实验设计,研究了提取液pH、提取温度、提取时间和料液比对黑豆种皮中花青素提取得率的影响,优化了黑豆种皮中花青素的提取工艺。结果表明,供试因素对黑豆种皮花青素提取得率的影响顺序是:提取液pH>料液比>提取温度>提取时间;确定的黑豆种皮花青素最佳提取工艺条件为:提取液浓度为50%,pH2.00的乙醇水溶液,料液比为1:25(g:mL),提取温度为50℃,功率为400W超声波提取10min。在最佳组合条件下,黑豆种皮中花青素提取得率3.87mg/g。 On the basis of ultrasonic assisted extraction, the effects of extraction pH, extraction temperature, extraction time and solid-liquid ratio on the extraction rate of anthocyanin in black bean seed coat were studied by L9 (34) orthogonal design. Extraction of Anthocyanin from Black Peel. The results showed that the order of influencing factors on the extraction yield of anthocyanin from black bean seed coat was as follows: the pH value of extraction solution> the extraction temperature> the extraction time; the optimum extraction conditions of anthocyanin in black bean seed coat were as follows: The concentration of the extract was 50%, the aqueous solution of ethanol with pH2.00, the ratio of material to liquid was 1:25 (g: mL), the extraction temperature was 50 ℃ and the power was 400W for 10min. Under the optimal combination conditions, the extraction rate of anthocyanin in black bean seed coat was 3.87mg / g.