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目的:探讨DMSO对运动诱导的大鼠海马神经发生的影响。方法:将30只雄性5周龄SD大鼠随机分为对照组、运动组、DMSO+运动组,通过预先埋管方式,向DMSO+运动组动物侧脑室内注射DMSO,注射1小时后,运动组和DMSO+运动组动物进行跑台运动30min,运动方案为:5m/min,5min;8m/min,5min;11m/min,20min。1周后所有动物取材,采用免疫荧光染色技术和荧光显微镜,检测各组大鼠海马齿状回内新生细胞的数量。结果:各组动物每个脑切片海马齿状回内BrdU免疫阳性细胞数平均为:对照组89.92±12.77个,运动组119.19±19.94个,DMSO+运动组73.94±17.36个。DMSO+运动组的BrdU+细胞数量较运动组减少了37.96%,BrdU++NeuN+细胞数量减少了40.56%。结论:DMSO对运动诱导的大鼠海马神经发生有显著的抑制作用。
Objective: To investigate the effect of DMSO on exercise-induced hippocampal neurogenesis in rats. Methods: Thirty male Sprague-Dawley (SD) rats, 5 weeks old, were randomly divided into control group, exercise group and DMSO + exercise group. DMSO was injected intracerebroventricularly (DMSO) The animals in the DMSO + exercise group were subjected to treadmill exercise for 30 minutes. The exercise program was: 5 m / min for 5 min, 8 m / min for 5 min, and 11 m / min for 20 min. After 1 week, all the animals were harvested. Immunofluorescence staining and fluorescence microscopy were used to detect the number of newborn cells in hippocampal dentate gyrus in each group. Results: The mean number of BrdU immunoreactive cells in the hippocampal dentate gyrus of each group was 89.92 ± 12.77 in the control group, 119.19 ± 19.94 in the exercise group and 73.94 ± 17.36 in the DMSO + exercise group. The number of BrdU + cells in DMSO + exercise group decreased by 37.96% and the number of BrdU ++ NeuN + cells decreased by 40.56% compared with exercise group. Conclusion: DMSO can significantly inhibit the hippocampal neurogenesis induced by exercise in rats.