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目的:建立灵敏、准确的液相色谱/质谱法测定元胡止痛软胶囊中延胡索乙素的含量。方法:样品经甲醇提取后,采用Lichrospher~(TM)C.18柱(250mm×2.1mm,5μm)分离,甲醇-20mmol·L~(-1)乙酸铵溶液(70:30)为流动相。质谱采用电喷雾离子化(ESI)方式,正离子采集,以选择离子(SIR)方式进行定量测定。结果:延胡索乙素的的线性范围为2.0～10.0ng·ml~(-1),平均加样回收率为98.1%,RSD为0.78%。结论:方法简便、灵敏、准确,可用于元胡止痛软胶囊中延胡索乙素的含量测定。
Objective: To establish sensitive and accurate liquid chromatography/mass spectrometry for the determination of tetrahydropalmatine in Yuanhu Zhitong soft capsules. Methods: The samples were extracted with methanol and separated by LichrospherTM C.18 column (250mm×2.1mm, 5μm). Methanol-20mmol·L -1 ammonium acetate solution (70:30) was used as the mobile phase. Mass spectrometry was performed using electrospray ionization (ESI), positive ion capture, and selective ion (SIR) measurements. Results: The linear range of tetrahydropalmatine was 2.0-10.0 ng·ml-1, the average recovery was 98.1%, and the RSD was 0.78%. Conclusion: The method is simple, sensitive and accurate. It can be used for the determination of Tetrahydropalmatine in Yuanhu Zhitong Soft Capsule.