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目的探讨肺腺样囊性癌(PACC)中Myb蛋白表达及其与预后的关系,p16蛋白表达及其与HPV感染状况的关系。方法收集北京协和医院2000—2014年间PACC共27例,采用免疫组化En Vision法检测其Myb、p16的表达,并对p16阳性细胞数>40%的病例用原位杂交方法进行HPV检测。结果 27例PACC,男女之比为8∶19,年龄24~74岁,平均年龄51岁;24例为肺原发性,3例为肺转移性。肺原发性病例中,18例随访1~132个月,其中5例复发或转移,1例死亡;6例失访。19例Myb(+)(79.2%),26例p16(+)(100%),对p16阳性细胞数>40%的11例加做HPV ISH均为(-)。无复发和转移组Myb阳性率(66.7%,8/12)低于复发或转移组(100%,8/8),原发性PACC组Myb阳性率(76.2%,16/21)低于转移性PACC组(100%,3/3),差异不显著(P>0.05)。结论大多数PACC中Myb为(+),但可能与预后无关。虽然p16也呈(+),但未检测到HPV感染,推测其可能不是PACC中p16蛋白阳性的原因。
Objective To investigate the expression of Myb protein in lung adenoid cystic carcinoma (PACC) and its relationship with prognosis, the relationship between p16 protein expression and HPV infection. Methods 27 cases of PACC were collected from Peking Union Medical College Hospital from 2000 to 2014. The expression of Myb and p16 was detected by immunohistochemical En Vision method. HPV was detected by in situ hybridization in cases with p16 positive cells> 40%. Results 27 cases of PACC, male to female ratio of 8:19, aged 24 to 74 years, mean age 51 years; 24 cases of primary pulmonary and 3 cases of lung metastasis. Among the primary pulmonary cases, 18 cases were followed up for 1 to 132 months, of which 5 were recurrent or metastatic, 1 died and 6 were lost. Nineteen cases of HPV ISH were (-) in 19 cases of Myb (+) (79.2%), 26 cases of p16 (+) (100%) and more than 40% of p16 positive cells. The positive rates of Myb in non-recurrence and metastasis group (66.7%, 8/12) were lower than those in recurrence or metastasis group (100%, 8/8) Sex PACC group (100%, 3/3), the difference was not significant (P> 0.05). Conclusions Myb is (+) in most PACC, but may not be related to prognosis. Although p16 was also (+) but no HPV infection was detected, it is speculated that it may not be the cause of p16 protein positive in PACC.