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采用高效液相色谱法建立抗癌散乙酸乙酯部位指纹图谱。利用硅胶层析进行纯化分离,以Kromasil 100A C18色谱柱,采用乙腈和磷酸水溶液为洗脱液进行梯度洗脱,流速为0.7 mL/min,柱温为35℃,检测波为323 nm。根据选择的色谱条件制订了不同批号抗癌散的指纹图谱。根据这8批抗癌散药材的分析结果可看出,指纹图谱相似度较高,该方法操作简便稳定,重复性好。说明不同批抗癌散药材的化学组成一致性较好,质量稳定。为抗癌散的进一步研究提供理论依据。
HPLC fingerprinting was used to establish fingerprints of ethyl acetate fractions. Silica gel chromatography was used for purification and separation. The separation was performed on a Kromasil 100A C18 column using acetonitrile and phosphoric acid aqueous solution as eluent. The flow rate was 0.7 mL/min, the column temperature was 35°C, and the detection wave was 323 nm. According to the selected chromatographic conditions, different batches of anticancer powder fingerprints were developed. According to the analysis results of these 8 batches of anti-cancer herbs, the similarity of the fingerprints is high, and the method is simple and stable with good repeatability. It shows that the chemical composition of different batches of anti-cancer herbs has good consistency and stable quality. For the further study of anti-cancer scattered to provide a theoretical basis.