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目的：以凝集素制成分子探针用于检测卵巢肿瘤细胞膜标志物，即凝集素受体，并与其他肿瘤标志物的检测进行比较。方法：卵巢癌变组织经匀浆、阶段离心、酶解、分子筛等处理得到卵巢癌变细胞膜糖肽，用凝集素亲和电泳法寻找卵巢癌细胞膜标志物，通过刀豆素提纯肿瘤标志物，给兔注射制备纯化的特异性兔抗体，再用ＥＬＩＳＡ检测卵巢肿瘤患者３８例血清中的凝集素受体浓度。结果：卵巢肿瘤恶性组血清凝集素受体浓度显著高于良性组（Ｐ＜０．００１），而且凝集素受体浓度随着卵巢癌细胞恶性程度及临床分期的增加而升高；用凝集素受体浓度值１１５．３Ｕ／ｍｌ或１７５．５９Ｕ／ｍｌ作为诊断卵巢癌的界值，其阳性率分别为９５．７％和７３．９％，高于同时用单克隆抗体ＣＡ１２５（６３．６％）、ＣＡ１５－３（７２．７％）、ＣＡ１９－９（５４．５％）的检测。结论：凝集素受体对卵巢癌的诊断有高度的敏感性和特异性，优于单克隆抗体。
OBJECTIVE: To construct a molecular probe of lectin for the detection of ovarian tumor cell membrane marker, lectin receptor, and to compare with the detection of other tumor markers. Methods: Ovarian cancerous tissues were treated with homogenate, phase centrifugation, enzymolysis and molecular sieve to obtain the glycopeptide of the cell membrane of ovarian cancer. The ovarian cancer cell membrane markers were screened by lectin affinity electrophoresis, and the tumor markers were purified by concanavalin Purified specific rabbit antibody was prepared by injection, and the concentration of lectin receptor in serum of 38 patients with ovarian tumor was detected by ELISA. Results: The concentration of serum lectin receptor in malignant ovarian tumor was significantly higher than that in benign ovarian cancer (P <0.001), and the concentration of lectin receptor increased with the malignant degree and clinical stage of ovarian cancer. The receptor concentration of 115.3U / ml or 175.59U / ml as the diagnostic value of ovarian cancer, the positive rates were 95.7% and 73.9%, respectively, higher than the same time with the monoclonal antibody CA125 (63.6 %), CA15-3 (72.7%), CA19-9 (54.5%). CONCLUSION: Lectin receptors are highly sensitive and specific for the diagnosis of ovarian cancer and are superior to monoclonal antibodies.