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本实验对人、兔睫状色素上皮(CPE)细胞进行体外培养获得成功。以免眼为对象,从取材、消化方法等方面对该细胞的体外培养进行了探讨。结果表明:采用机械方法剥离睫状上皮和小牛血清控制了的培养液能够培养出纯净的CPE细胞。首次将胶原酶用于该细胞的培养,能够完全消化残留玻璃体,提高了培养成功率(88%)。形态对比研究发现:培养的细胞内细胞器丰富,染色体复制与细胞有丝分裂功能增强,而表面微绒毛形态、细胞连接和早期黑色素等方面与未培养细胞保持一致。
In this experiment, human and rabbit ciliary epithelium (CPE) cells were cultured successfully in vitro. To avoid the eye as the object, from the material, digestion and other aspects of the cell culture in vitro were discussed. The results showed that pure cultured CPE cells could be cultured by mechanically stripping the culture fluid controlled by ciliary epithelium and calf serum. For the first time, collagenase was used in the culture of this cell to completely digest the residual vitreous and improve the success rate of culture (88%). Morphological contrast study found that: cultured cells organelles rich in chromosome duplication and mitosis enhanced function, while the surface microvilli morphology, cell adhesion and early melanin aspects consistent with the uncultured cells.