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目的采用RNA干扰与噬菌体肽库展示技术2种方法干预宫颈癌HeLa细胞CD59的表达,以探讨比较2种CD59低表达方式对HeLa细胞增殖与凋亡的影响。方法将10μg/mL的CD59短肽封条作用于HeLa细胞8 h后,联合CD59干扰质粒pSUPER-siCD59稳定转染HeLa细胞系,采用MTT法检测各组细胞增殖率的变化,采用TUNEL染色、annexin V-PE/7-AAD染色结合流式细胞术检测细胞凋亡。结果 CD59短肽封条组与pSUPER-siCD59质粒稳定转染细胞组HeLa细胞增殖明显弱于对照组,TUNEL及流式细胞术检测结果显示2组处理细胞均存在细胞凋亡现象,且短肽封条的效果要好于pSUPER-siCD59质粒。结论下调CD59表达能抑制其细胞增殖并促进其凋亡,且短肽封条效果要优于CD59 RNA干扰质粒。
OBJECTIVE: To investigate the effects of two low expression of CD59 on the proliferation and apoptosis of HeLa cells by using two methods of RNA interference and phage display peptide library technology to investigate the expression of CD59 in cervical cancer HeLa cells. Methods After HepG2 cells were treated with 10 μg / mL CD59 for 8 h, HeLa cells were stably transfected with CD59 interference plasmid pSUPER-siCD59. The proliferation of HeLa cells was detected by MTT assay. TUNEL staining, annexin V Apoptosis was detected by -PE / 7-AAD staining and flow cytometry. Results The proliferation of HeLa cells was significantly weaker than that of the control cells in the CD59 short peptide-seal group and pSUPER-siCD59 plasmid-transfected cells. TUNEL and flow cytometry showed that there were apoptotic cells in both groups, The effect is better than pSUPER-siCD59 plasmid. Conclusion Down-regulation of CD59 expression can inhibit cell proliferation and promote apoptosis, and the effect of short peptide seal is better than CD59 RNA interference plasmid.