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以新疆野生苍耳叶为原料,利用超声波提取黄酮类化合物。在单因素试验的基础上,采用正交试验法,考察乙醇体积分数、料液比、超声温度、超声时间对总黄酮得率的影响,并用紫外可见分光光度法测定苍耳叶中总黄酮得率,最终确定最佳提取工艺条件为乙醇体积分数50%、料液比1:50、超声温度30℃、超声时间30min。在该最佳条件下,总黄酮得率为3.10mg/g,该方法采用全物理过程,无任何污染,是提取苍耳叶中黄酮类物质的有效途径。
Wild Xinjiang cocklebur leaves as raw materials, the use of ultrasonic extraction of flavonoids. On the basis of single-factor test, orthogonal test was used to study the effects of ethanol volume fraction, solid-liquid ratio, ultrasonic temperature and ultrasonic time on the yield of total flavonoids. The total flavonoids The optimal extraction conditions were as follows: ethanol volume fraction 50%, solid-liquid ratio 1:50, ultrasonic temperature 30 ℃, ultrasonic time 30 min. Under the optimal conditions, the yield of total flavonoids was 3.10mg / g. This method uses the whole physical process without any pollution and is an effective way to extract flavonoids from the cocklebur leaves.