论文部分内容阅读
背景与目的:三肽化合物酪丝缬肽(tyroservaltide,YSV)对实验性肝癌具有明显抑制作用,本研究观察YSV对小鼠黑色素瘤细胞B16-F10侵袭和转移的抑制作用。方法:MTT法检测YSV对B16-F10的细胞毒作用;利用基质胶Matrigel研究YSV对细胞粘附能力的影响;用Transwell小室侵袭模型研究YSV对肿瘤细胞侵袭能力的改变;经C57/BL6小鼠尾静脉注射B16-F10细胞,建立人工肺转移模型,观察YSV对B16-F10肺转移的影响;免疫组化方法观察YSV对细胞间粘附分子(intercellularadhesionmolecule-1,ICAM-1)在肺组织中表达水平的影响。结果:100μg/mlYSV作用48h对B16-F10细胞的增殖抑制率为24.36%;作用24h对B16-F10细胞在Matrigel胶上的粘附抑制率为36.51%;10μg/mlYSV作用48h对B16-F10细胞的侵袭抑制率为36.53%;640μg·(kg·d)-1YSV抑制B16-F10的肺转移,抑制率为62.21%;YSV组ICAM-1的组织量明显少于生理盐水组。结论:YSV具有抑制B16-F10生长和侵袭转移的作用。
BACKGROUND & OBJECTIVE: The tripeptide tyroservaltide (YSV) has a significant inhibitory effect on experimental liver cancer. This study investigated the inhibitory effect of YSV on the invasion and metastasis of B16-F10 in mouse melanoma cells. Methods: The cytotoxic effect of YSV on B16-F10 was detected by MTT assay. The effect of YSV on cell adhesion was investigated by Matrigel. The invasion of YSV was evaluated by Transwell chamber invasion assay. B16-F10 cells were injected into tail vein to establish an artificial lung metastasis model to observe the effect of YSV on lung metastasis of B16-F10. The effect of YSV on the expression of intercellular adhesion molecule-1 (ICAM-1) in lung tissue was observed by immunohistochemistry Effect of expression level. Results: The proliferation inhibition rate of B16-F10 cells treated with 100μg / ml SYV for 48h was 24.36%. The inhibition rate of B16-F10 cells on Matrigel was 36.51% after treated with 100μg / ml SYV for 48h, The invasion inhibition rate was 36.53% .6YSF inhibited the lung metastasis of B16-F10 with the inhibition rate of 62.21%. The tissue volume of ICAM-1 in YSV group was significantly less than that of saline group. Conclusion: YSV can inhibit the growth, invasion and metastasis of B16-F10.