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目的检测假手术和脑缺血再灌注大鼠脑组织小分子RNA的差异表达。方法选取假手术组(F)和脑缺血再灌注组(B)大鼠海马脑组织,提取小分子RNA,应用Solexa高通量测序技术进行深度测序,通过信息分析获得miRNA的表达情况。结果 F组和B组分别获得6 833 331和6 407 399个序列,平均长度均为22 nt。将原始数据与mjRbase、Genbank和Rfam(9.1)数据库进行比对和分类注释。在F组和B组,miRNA总数分别占小分子RNA总数的76.77%和75.22%,两组间共有32种差异表达的mjRNA,预测出新的候选miRNA 130条。结论 Solexa测序是一种快速、全面地研究和发现小分子RNA的重要手段,筛选出的差异表达及候选miRNA可能参与脑缺血再灌注的发展。
Objective To detect the differential expression of small molecule RNA in brain tissue of sham operation and cerebral ischemia reperfusion rats. Methods The hippocampal tissue of sham-operation group (F) and cerebral ischemia-reperfusion group (B) were selected to extract small RNA. Solexa high-throughput sequencing was used to perform deep sequencing. The miRNA expression was obtained by information analysis. Results There were 6 833 331 and 6 407 399 sequences in group F and group B, respectively, with an average length of 22 nt. The original data is aligned with and annotated with the mjRbase, Genbank and Rfam (9.1) databases. In group F and group B, the total number of miRNAs accounted for 76.77% and 75.22% of the total number of small RNAs respectively. There were 32 differentially expressed mjRNAs between the two groups, and 130 new candidate miRNAs were predicted. Conclusion Solexa sequencing is a rapid and comprehensive method for the research and discovery of small RNAs. The differentially expressed candidate miRNAs may be involved in the development of cerebral ischemia-reperfusion.