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目的 :建立一种准确度和灵敏度高 ,重现性好的汉防已甲素高效液相色谱测定法。方法 :采用ODSC18( 4.6mm× 1 50mm)为分析柱 ,流动相 :十二烷基磺酸钠 乙腈 0 .1mol/L磷酸盐缓冲液 (pH2 ) ( 0 .1 5∶50∶50 ) ,检测波长24 0nm。结果 :含量测定汉防已甲素在 0 .0 6～ 0 .3 0 μg线性关系良好 ,回收率 99.9% ,RSD为 1 .66%。 结论 :建立的含量测定方法简便、准确、灵敏。
Objective: To establish a high accuracy and high sensitivity, reproducibility of ciprofloxacin HPLC assay. Method: ODSC18 (4.6mm×150mm) was used as the analytical column. The mobile phase was: sodium dodecylsulfate acetonitrile 0.1 mol/L phosphate buffer (pH2) (0.15:50:50). Wavelength 24 nm. RESULTS: The linearity of the content of anticancerin was 0.06 to 0.30 μg. The recovery rate was 99.9% and the RSD was 1.66%. Conclusion : The established method for determination of content is simple, accurate and sensitive.