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利用高粱转换系与具相同核背景不同细胞质的Ａ＿１Ｔ＿ｘ６１６、Ａ＿２Ｔ＿ｘ６１６、Ａ＿３Ｔ＿ｘ６１６不育系杂文，对Ｆ＿１植株花粉育性调查表明，三种不同不育细胞质之间存在着明显的差异，Ａ＿１与Ａ＿２型不育细胞质之间差异较小。Ａ＿１、Ａ＿２个与Ａ＿３型不育细胞质之间差异较大，Ａ＿３型不育细胞质的育性反应与Ａ＿１和Ａ＿２型不育细胞质的育性反应截然不同。三种不育细胞质对Ｆ＿１代主要农艺性状的影响无明显差异，Ａ＿２型不育细胞质可以在高粱杂交种生产中加以利用。找到了恢复Ａ＿３型细胞质的恢复源和同时能恢复Ａ＿１、Ａ＿２、Ａ＿３型三种不育细胞质的恢复材料。三种不育细胞质育性恢复机制不同，细胞质对育性反应起决定性作用。
The pollen fertility of F_1 plants was investigated using sorghum transformation lines and A_1T_x616, A_2T_x616 and A_3T_x616 male sterile lines with different cytoplasmic nuclei with the same nuclear background. The results showed that there were significant differences among the three different sterile cytoplasms. However, A_1 and A_2 The difference between the cytoplasm of fertility is smaller. A_1, A_2 and A_3-type sterile cytoplasm differences between the larger A_3-type sterile cytoplasm fertile reaction and A_1 and A_2 sterile cytoplasm fertile reaction is completely different. The effect of three kinds of sterile cytoplasm on the main agronomic traits of F1 was no significant difference. The type A_2 sterile cytoplasm could be used in the production of sorghum hybrid. The recovery materials for recovering A_3 cytoplasm and recovering three kinds of sterile cytoplasm A_1, A_2 and A_3 were found at the same time. The fertility restoration mechanisms of three kinds of sterile cytoplasm are different, and the cytoplasm plays a decisive role in the fertility response.