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为了阐明H2O2对水稻白叶枯病菌(Xanthomonas oryzaepv.oryzae,Xoo)过氧化氢酶(CAT)相关基因(crg)表达的诱导作用,本研究定量分析了在水稻细胞-Xoo互作体系及其加入H2O2清除剂CAT后H2O2产量和crg表达;外源添加H2O2后的病菌生长和crg表达。结果表明:在互作条件下,H2O2含量稳定增加,10 h可达到峰值;在互作6 h时crg显著地被诱导表达;加入CAT显著地降低了H2O2含量和crg表达;在外源H2O2胁迫条件下,H2O2以浓度效应的方式影响病菌增殖,显著地诱导了catB和srpA表达。因此,Xoo-水稻互作导致了H2O2的发生。无论是互作产生的还是外源的H2O2均显著地诱导了Xoocrg表达,从而活化了H2O2降解途径。
In order to elucidate the induction of catalase (CAT) -related gene (crg) expression induced by H2O2 in Xanthomonas oryzaepv. Oryzae (Xoo), this study quantitatively analyzed the effect of H2O2 The H2O2 production and the expression of crg after H2O2 scavenger CAT were increased. The growth of germs and the expression of crg after the addition of H2O2 were also observed. The results showed that the content of H2O2 increased steadily and reached the peak at 10 h under the intercritical conditions. The expression of crg was induced significantly at 6 h of intercrossing. The addition of CAT significantly reduced the content of H2O2 and the expression of crg. Under the condition of exogenous H2O2 stress , H2O2 affected the proliferation of pathogenic bacteria in a concentration-dependent manner and significantly induced the expression of catB and srpA. Therefore, Xoo-rice interaction led to the occurrence of H2O2. Both the interaction and exogenous H2O2 induced Xoocrg expression significantly, which activated the H2O2 degradation pathway.