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目的:探讨HPV感染引起宫颈病变后,宿主外周血T细胞亚群与细胞因子变化情况。方法:PCR扩增后,经核酸杂交,分高危组、低危组和正常对照组;BD-流式细胞仪检测外周血CD3+、CD4+、CD8+及IL-2的含量。结果:CD3+T细胞:三组相比无显著性差异(P>0.1);CD4+T细胞:A、B组低于C组,差异有显著性(P<0.005)且A组低于B组(P<0.025);CD8+T细胞:A组低于B、C组差异有高度显著性(P<0.005),B、C组差异无显著性(P>0.25);CD4+/CD8+比值:A组低于C组,差异有显著性。IL-2:A、B组低于C组,差异有显著性(P<0.005),A、B组差异无显著性。结论:免疫功能低下是HPV不易治愈和反复的主要因素。
Objective: To investigate the changes of T cell subsets and cytokines in peripheral blood of patients with HPV-induced cervical lesions. Methods: The levels of CD3 +, CD4 +, CD8 + and IL-2 in peripheral blood were detected by BD-flow cytometry after nucleic acid hybridization, high risk group, low risk group and normal control group. Results: CD3 + T cells: there was no significant difference between the three groups (P> 0.1); CD4 + T cells: A, B group was lower than C group, the difference was significant (P <0.05). There was no significant difference between group B and C (P> 0.05). The ratio of CD4 + / CD8 +: CD8 + T cells in group A was significantly lower than group B and C A group was lower than C group, the difference was significant. IL-2: A, B group was lower than C group, the difference was significant (P <0.005), A, B group no significant difference. Conclusion: Immunocompromised HPV is not easy to cure and repeated the main factors.