【摘 要】
：
The gdhA genes of IRC-3 GDH-strain and IRC-8 GDH+ strain were cloned,and they both successfully complemented the nutritional lesion of an E.coli glutamate auxot
【机 构】
：
Shanghai Institute of Plant Physiology
论文部分内容阅读
The gdhA genes of IRC-3 GDH-strain and IRC-8 GDH+ strain were cloned,and they both successfully complemented the nutritional lesion of an E.coli glutamate auxotroph,Q100 GDH-.However,the gdhA gene from the mutant IRC-8 GDH+ strain failed to complement the glutamate deficiency of the wild type strain IRC-3.The gdhA genes of the wild type and mutant origin were sequenced separately.No nucleotide difference was detected between them.Further investigations indicated that the gdhA genes were actively expressed in both the wild type and the mutant.Additionally,no GDH inhibitor was found in the wild type strain IRC-3.It is thus proposed that the inactivity of GDH in wild type is the result of the deficiency at the post-translational level of the gdhA expression.Examination of the deduced amino acid sequence of Bacillus licheniformis GDH revealed the presence of the motifs characteristic of the familyⅠ-type hexameric protein,while the GDH of Bacillus subtilis belongs to family II.
其他文献
Mixed-conducting oxygen permeable membranes represent a class of novel ceramic membranes, which exhibit mixed oxygen ionic and electronic conductivities. At hig
通过对深基坑支护基本要求分析，并结合以往经验研究，提出深基坑工程的质量控制要点以及深基坑监测等方面的具体内容，提出了如何加强施工中各单位紧密配合以及各工序合理安排问题
本文主要阐述建筑施工的过程，简单介绍了建筑施工过程中遇到的问题，进行了详细的分析，并且从建筑施工质量管理的高度出发，提出几点提高施工质量的方法。
The studies of the oxgen and carbon isotopes of the rainwater in Guilin area, the drip water and the present carbonate deposit in Panlong cave of Guilin show th
现代的工程建筑既要追求工程的质量又要追求的速度，裂缝的产生则给施工的时机选择带来了制约作用。但是，通过后浇带的技术控制可以有效缓解因混凝土结构出现的施工裂缝。本文将
The Co/Cu/Co sandwiches with a semiconductor Si buffer layer were prepared by high vacuum electron-beam evaporation. The influence of the Si buffer layer with d
建筑工程不仅是一项对人工、材料、能源的消耗都十分大的工程项目，也是一款投资者通过进行巨大的投资后对获得理想的、满足适用要求很高的的工程产品。但建筑工程在施工的过程
In this article, we propose sharpening the gain of the chaotic annealing neural network to solve 0- 1 constrained optimization problem. During the chaotic annea
电网企业采用信息化手段是实现“一强三优”目标的必要举措之一.以文登市供电公司为例,阐述了电网企业实施营销信息化的必要性,并提出了实现营销信息化的三个步骤及实现途径.
The extraction behavior of uranium(VI) from thiocyanate medium with dibenzo-18-crown-6 (DBC) in nitrobenzene and the effects of alkali cations on the extraction