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目的 :探讨不同孕期人乳头状瘤病毒 ( HPV)亚临床感染及母婴垂直传播情况。方法 :采用 PCR技术检测 3 0例孕早期、 4 2例孕中期、 3 2例孕晚期妇女的宫颈分泌物、母血、绒毛或胎盘、羊水、脐血及新生儿咽部抽吸物标本 HPV- 6、 11、 16、 18型 DNA。结果 :早孕期 :5例宫颈分泌物 HPV阳性 ( 5 /3 0 ) ,7例母血 HPV阳性 ( 7/3 0 ) ,3例绒毛 HPV阳性 ( 3 /3 0 ) ;中孕期 :12例宫颈分泌物 HPV阳性 ( 12 /4 2 ) ,11例母血 HPV阳性 ( 11/4 2 ) ,9例胎盘 HPV阳性 ( 9/4 2 ) ;另 2 2例羊水标本中 ,仅 1例 HPV阳性 ( 1/2 2 ) ;晚孕期 :2 3例宫颈分泌物 HPV阳性( 2 3 /3 2 )、 18例母血 HPV阳性 ( 18/3 2 ) ,12例胎盘 HPV阳性 ( 12 /3 2 ) ;另 3 1例羊水标本中 ,4例 HPV阳性 ( 4 /3 1) ,3 1例脐血标本中 ,12例 HPV阳性 ( 12 /3 1)。分析羊水、脐血与母血 HPV感染的相对危险度高于宫颈分泌物 ,而产时新生儿咽部抽吸物与宫颈分泌物 HPV感染的相对危险度高于母血。结论 :在整个孕期中 ,孕晚期的HPV亚临床感染明显。孕期 HPV亚临床感染的母婴传播不但可经产道直接接触传播 ,还可经羊水、胎盘发生宫内传播 ,其宫内传播率的高低主要与母血 HPV感染相关。新生儿脐血的 HPV感染主要与母血感染相关 ,而产时新生儿咽部的 HPV
Objective: To investigate the subclinical infection of human papillomavirus (HPV) and the vertical transmission of mother-infant in different pregnancy. Methods: The cervical secretions, maternal blood, villi or placenta, amniotic fluid, umbilical cord blood and neonate aspirate specimens of 30 pregnant women in the first trimester, 42 pregnant women in the second trimester were detected by PCR. HPV - 6, 11, 16, 18 DNA. Results: In the first trimester, 5 cases of cervical secretions were HPV positive (5/30), 7 cases of maternal blood HPV positive (7/3 0), 3 cases of villus HPV positive (3/3 0) (12/42), HPV positive (11/42) in 11 maternal blood samples and 9 (9/42) HPVs in 9 placenta samples. Only 1 HPV was positive in the other 22 samples of amniotic fluid In the second trimester of pregnancy, 23 cases were HPV positive (23/32), 18 were maternal blood (18/3 2), and 12 cases were positive for placenta (12/3 2). Of the 31 other cases of amniotic fluid samples, 4 were HPV positive (4/3 1), of which 12 were HPV positive (12/3 1) in 31 cord blood samples. Analysis of amniotic fluid, umbilical cord blood and maternal blood HPV infection relative risk higher than cervical secretions, and neonatal aspartate aspartate aspirate and cervical secretions HPV infection relative risk higher than maternal blood. CONCLUSIONS: Subclinical HPV infection during the third trimester was evident throughout pregnancy. Pregnancy HPV subclinical infection of mother-to-child transmission can not only be transmitted directly through the birth canal, but also by amniotic fluid, intrauterine transmission of placenta, the level of intrauterine transmission is mainly associated with maternal blood HPV infection. Neonatal cord blood HPV infection is mainly associated with maternal blood infection, while the production of neonatal pharynx HPV