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目的 研究葛根素口服制剂对在体大鼠心肌和离体新生大鼠心肌细胞的保护作用。方法 实验大鼠随机分为空白对照组、阳性对照组 (造模前尾 iv 0 .0 335 g/ kg葛根素注射液 )和葛根素口服制剂高、低剂量组 ( ig 0 .8,0 .4 g/ kg,连续 6 d) ,以 1U/ kg垂体后叶素 ( Pit)诱导大鼠心肌缺血 ,记录尾 iv Pit前及注射后 5 ,15 ,30 s和 2 ,5 ,10 min大鼠心电图变化 ;显微镜观察心肌细胞形态学变化及测定不同时间点培养液中乳酸脱氢酶 ( L DH)、肌酸激酶 ( CK)和α-羟丁酸脱氢酶 (α- HBDH )的活性。结果 葛根素口服制剂 ig给药对心肌缺血大鼠的心电图 T波、P- R间期、Q- T间期变化有明显的改善 ;能对抗缺氧状态下离体新生大鼠心肌细胞形态学和酶学的异常变化。结论 葛根素口服制剂对缺氧大鼠心肌细胞有保护作用
Objective To study the protective effect of puerarin oral preparation on myocardium and isolated neonatal rat cardiomyocytes in vivo. Methods Rats were randomly divided into blank control group, positive control group (iv 0. 0 335 g/kg puerarin injection before modeling) and puerarin oral high and low dose group (ig 0.8). At 4 g/kg for 6 consecutive days, myocardial ischemia was induced in rats with 1 U/kg pituitrin (pit) and recorded before and after iv iv pit 5, 15, 30 s and 2, 5, and 10 min. Electrocardiogram changes; microscopic observation of myocardial cell morphological changes and determination of lactate dehydrogenase (LDH), creatine kinase (CK), and α-HBDH activity in culture fluids at different time points . Results The ig administration of puerarin oral administration of ig administered electrocardiogram T-wave, P-R interval, Q-T interval changes of myocardial ischemia in rats was significantly improved; can protect against hypoxia in neonatal rat cardiomyocyte morphology Abnormal changes in learning and enzymology. Conclusion Puerarin oral preparation has protective effects on hypoxic rat cardiomyocytes