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用ＤＥＡＥ－纤维素和肝素－Ｓｅｐｈａｒｏｓｅ４Ｂ层析法部分纯化了大鼠肝酪蛋白激酶Ⅱ（ＣＫⅡ）；研究了５种抗血小板药对ＣＫⅡ活性的影响。结果表明，肝素对ＣＫⅡ有强烈的抑制作用，其ＩＣ５０为０．８８ｍｍ·Ｌ－１；蝙蝠葛碱、川芎嗪对ＣＫⅡ有明显的抑制作用（Ｐ＜０．０１），但较肝素的作用弱得多；汉防己碱对ＣＫⅡ活性没有明显影响；而潘生丁能使ＣＫⅡ活性明显升高，在一定范围内呈剂量依赖关系。这些结果可能对研究ＣＫⅡ是否在血小板的聚集和活化中起某些作用有重要的参考价值。
Rat hepatic casein kinase Ⅱ (CKⅡ) was partially purified by DEAE-cellulose and heparin-Sepharose 4B chromatography. The effects of five antiplatelet agents on CKⅡ activity were studied. The results showed that heparin had a strong inhibitory effect on CKⅡ with an IC50 of 0.88mm · L-1. Dauricine and ligustrazine significantly inhibited CKⅡ (P <0.01), but their effects were weaker than that of heparin Much more; tetrandrine had no obvious effect on CKⅡ activity; while dipyridamole could obviously increase CKⅡ activity in a dose-dependent manner. These results may be important for investigating whether CKⅡ plays a role in platelet aggregation and activation.